In this call, the IMTM-HTS facility offers long term scientific expertise in live-cell based screening and high-content analysis of reporter or stably transfected cells for monitoring of cytoplasmatic, nuclear or nucleolar targets and processes; e.g., DNA damage and repair in fixed and live cells. The HTS system consists of three robotic arms, automatic incubators, liquid handlers, readers for fluorescence, luminescence, absorbance or ionizing radiation, X-Ray irradiator, and confocal microscopes. An essential part of such screening is available image analysis by advanced software algorithms at our computational cluster.
In this call, the IMTM-HTS facility offers long term scientific expertise in live-cell based screening and high-content analysis. The IMTM facility can provide assays directed towards a wide range of targets involved in vital biological processes such as DNA damage response or the cell cycle progression. The assays are based on imaging of reporter or stably transfected cells and monitoring of diverse processes such as signal accumulation in a specific compartment or cytoplasmic to nuclear translocation. Our instrumentation enables live-cell imaging and time-lapse microscopy followed by a high-content analysis of acquired images.
IMTM has a modular HTS platform that allows testing in BSL3 and BSL2+ environments, screening in combination with ionizing radiation (X-rays), mass spectrometry, high-content analysis and other technologies. The screening assays provide hits for downstream drug research and development. The HTS/HCA screening platform is based on a state-of-art robotic system provided by HighResBiosolutions Ltd. The system consists of three robotic arms, automated incubators, liquid handlers for microliter and nanoliter volumes, sealers, de-sealers, centrifuges and readers for fluorescence, luminescence, absorbance and ionizing radiation. Also integrated with the system are wide-field or spinning disc confocal microscopes (Operetta, Yokogawa CV7000) equipped with software tools for image analysis and data evaluation. The IMTM screening facility can offer assays for screening of biologically active compounds and anticancer agents, assays for identification of adenosine receptor agonists and antagonists as well as mapping of the most significant signaling pathways (e.g., Hedgehog, Wnt, p53, KRAS, RAGE) via phosphorylation and reporter assays. In addition to mammalian cells, IMTM laboratories are fully equipped for the screening of antimicrobial activity in G+/G- strains including drug-resistant bacteria, mycobacterial strains, yeasts, filamentous fungi, viruses and parasites under BSL2 and at limited capacity under BSL3 conditions.
100,000 (full commercial set) of the EU-OPENSCREEN ERIC compound library
Please note that this project includes the possibility of re-screening during potential chemical optimization under the medicinal chemistry call starting in 2021 (tentative date).
The applicant is expected to have a project with clearly defined objectives. The applicant needs to show preliminary data confirming the reproducibility of the biological assay or demonstrate a measurable change in phenotype of the cell line used. Before the transfer of any cell line to our facility, the applicant has to prove that the cell line is free of mycoplasma. The applicant should effectively communicate or be temporarily present on the screening site in order to facilitate the adaptation to automated screening and provide key bespoke reagents.
As specifics of the assay transfer procedure may vary between partner sites, the applicant and the individual sites will agree on the appropriate steps and logistics together.
Cell-based assays using cellular systems
drug discovery, small molecules, high-throughput screening, cell-based assays
June-01-2019 (20:00 CET)
September-30-2019 (20:00 CET)
Scientific contact: firstname.lastname@example.org
Technical contact: email@example.com
Machine/Methods contact: firstname.lastname@example.org, email@example.com